Vol 3-1 Commentary

Commentary on the "Co-Crystal of Tramadol-Celecoxib in Patients with Moderate to Severe Acute Post-Surgical Oral Pain: A Dose-Finding, Randomised, Double-Blind, Placebo- and Active-Controlled, Multicentre Phase II Trial". Implications for Cardiovascular Safety

Sebastián Videla1*, Neus Gascón2, Carlos Plata-Salamán2

1Clinical Research Support Unit, Clinical Pharmacology Department, Bellvitge University Hospital/IDIBELL, Barcelona, Spain

2Medical Sciences Area, ESTEVE Pharmaceuticals S.A., Barcelona, Spain

DOI: 10.29245/2578-3025/2019/1.1163 View / Download Pdf
Vol 3-1 Commentary

Commentary: Atherosclerosis, Analysis of the eNOS (T786C) Gene Polymorphism

Kleber Santiago Freitas e Silva*

Biological Sciences Institute, Federal University of Goiás, Brazil

DOI: 10.29245/2578-3025/2019/1.1166 View / Download Pdf
Vol 3-1 Mini Review

Electron Tomography Contributing to Ultrastructural Research in Vascular Biology and Transfusion Medicine

Josef Neumüller1,2, Thomas Wagner3*

1Blood Donation Center of the Austrian Red Cross for Vienna, Lower Austria and Burgenland

2Center for Anatomy and Cell Biology, Department for Development Biology, Medical University of Vienna

3Department of Blood Group Serology and Transfusion Medicine, Medical University of Graz, Austria

In this review, the technique of electron tomography is shortly summarized. Alignment procedures on the electron microscope are described. New techniques such as the focused ion beam tomography, the block face scanning tomography and the array tomography are mentioned. Application of these methods for vascular research, concerning endothelium-specific Weibel-Palade bodies in cell cultures from human umbilical vein endothelial cells and endothelial progenitor cells as well as about cellular communications between the endothelium and the subendothelium are referred. Platelets are important players in the interaction with the endothelium in thrombosis and hemostasis after transfusion, therefore electron tomographic investigations of platelet organelles as well as platelet microparticles are mentioned. Illustrations from our own investigations are added.

ART: Algebraic Reconstruction Technique; ADAMTS-13: A Disintegrin And Metalloproteinase with A Thrombospondin Type 1 Motif, Member 13; CCD Camera: Charge Coupled Device Camera; DAB: 3,3'-Diaminobenzidine; DART: Discrete Algebraic Reconstruction Technique; DTS: Dense Tubular System; EM: Electron Microscope; EPC: Endothelial Progenitor Cells; ET: Electron Tomography; FVIII: Factor VIII Blood Clotting Protein; FIB-SEM: Focused Ion Beam Scanning Electron Microscope; FITC: Fluorescein-Isothiocyanate; HUVEC: Human Umbilical Vein Endothelial Cells; LSM: Laser Confocal Scanning Microscope; MIT: Mitochondria; MTC: Microtubular Coil; OCS: Surface-Connected Open Canalicular System; PLT: Platelets; PLTC: Platelet Concentrates; PMP: Platelet Microparticles; ROI: Region Of Interest; SBEM: Serial Block Face Scanning Electron Microscopy; SIRT: Simultaneous Itinerative Reconstruction Technique; STEM: Scanning Transmission Electron Microscopical Device; TEM: Transmission Electron Microscope; TGN: Trans Golgi Network; TRITC: Tetramethylrhodamine-Isothiocyanate; vWF: von Willebrand Factor; WPB: Weibel Palade Bodies; WPB: Method: Weighted Back Projection Method.

DOI: 10.29245/2578-3025/2019/1.1165 View / Download Pdf